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Determination and Characterization of Viruses, Harmful to Stem Tissue of Pome Fruit Trees by Biological, Serological and Molecular Techniques

Identifier : Catalog : URI
Entry : http://traglor.cu.edu.tr/objects/objectFile/HqDqa59V-892013-23.pdf


Title : Turkish Yumuşak Çekirdekli Meyve Ağaçlarında Gövde Zararlanmalarına Neden Olan Viral Etmenlerin Biyolojik, Serolojik ve Moleküler Yöntemlerle Saptanması ve Karakterizasyonu
English Determination and Characterization of Viruses, Harmful to Stem Tissue of Pome Fruit Trees by Biological, Serological and Molecular Techniques
German Bestimmung und Charakterisierung von Viren, schädliche Wirkung auf das Gewebe Pome Obstbäume Biologische, serologische und molekulare Verfahren Stem


Language : Turkish Turkish



Descriptions : Turkish Bu çalışma Adana, Kahramanmaraş, Mersin, Niğde ve Osmaniye illerinde bulunan elma, armut ve ayva alanlarında 2006-2009 yılları arasında yürütülmüştür. Çalışmanın amacı; yumuşak çekirdekli meyve ağaçlarında zararlı Elma Gövde Çukurlaşma (Apple stem grooving capillovirus: ASGV) ve Elma Gövde Yivleşme (Apple stem pitting foveavirus: ASPV) virüs hastalıklarının saptanması, elde edilen izolatların karakterizasyonu ve bazı elma çeşitlerinin bu hastalıklara karşı reaksiyonlarının belirlenmesidir. Sürvey çalışmaları sonucunda 134 bahçeden alınan 619 elma, armut ve ayva örneğinin %18,41 oranında bu hastalık etmenleri ile enfekteli olduğu ELISA testi ile belirlenmiştir. Hastalık etmenlerinin bulunma oranı sırasıyla ASPV % 10,34, ASGV %5,49 ve ASPV+ASGV % 2,58, konukçu türlerin enfeksiyon oranları ise armut % 19,35, elma %18,33 ve ayva %17,39 şeklinde tespit edilmiştir. Bu hastalık etmenlerinin varlığı moleküler ve biyolojik yöntemlerle de doğrulanmıştır. Sürvey alanlarında yapılan makroskobik gözlemler sonucunda bu hastalıkların konukçuları üzerinde bazı belirtiler meydana getirdiği ve özellikle ASPV’nin ayva ve armut üzerinde ekonomik zarara neden olduğu tespit edilmiştir. Karakterizasyon çalışmaları sonucunda ASPV ve ASGV hastalık etmenlerinin konukçularına göre farklı biyolojik karakterlere sahip oldukları, karışık enfeksiyon halinde bulundukları durumlarda daha tahripkar seyrettikleri, fakat moleküler düzeyde izolatlar arasında büyük bir fark olmadığı belirlenmiştir. Çeşit reaksiyon denemesi çalışmalarında, bu hastalık etmenlerinin bazı ticari elma çeşitlerinin gövde çapında ve odun dokusu kuru ağırlığında azalmaya, dal açılarında ise genişlemeye neden oluduğu tespit edilmiştir. Ayrıca bu etmenlerin bazı ticari elma çeşitlerinde floem nekrozu ve dallarda şişkinlik şeklinde belirtilere de yol açtığı gözlenmiştir.
English This study was carry out in Adana, Kahramanmaraş, Mersin, Niğde and Osmaniye provinces between years of 2006 and 2009. The aim of the study was determination and characterization of Apple Stem Grooving Capillovirus (ASGV) and Apple Stem Pitting Foveavirus (ASPV) on pome fruits and evaluation of the reactions of some commertial cultıvars to the diseases. During the survey, 619 samples were collected from 134 orchards and, 18,41 % of the samples were founded as ASPV, ASGV or ASPV+ASGV infected by ELISA test. The incidence of the diseases were determined as 10,34 % ASPV, 5,49 %ASGV and 2,58 % ASPV+ASGV. The infection rate of the species were determined as 19,35, 18,33 and 17,39 % in pear, apple and in quince, respectively. The presence of these diseases was confirmed also by molecular and biological means. By the macroskobik observation of the diseases in survey area the study showed that, diseases are inducing some symptoms on the host plant especialy ASPV was resulting economical damage on quince and pear. ASPV and ASGV were determined that can have different biological activity on their host plants according their origin’s. Impact of the diseases agents were very valuable in case of being mixed infection on the indicator plants. However, no any significant differences have been observed between isolates by the molecular characterisation. Moreover, the diseases were founded that they can reduce the trunk diameter and dryweight of the woody tissues, and increase the angle of the branches on some commertial cultivars. The floem necrosis and stem grooving symptoms on some succeptible commertial cultivars were observed in the reaction assay due to the ASPV and ASGV infections.
German Diese Studie wurde die Durchführung in Adana, Kahramanmaraş, Mersin, Niğde und Osmaniye Provinzen zwischen den Jahren 2006 und 2009. Das Ziel der Studie war die Bestimmung und Charakterisierung von Apple Stem Einstechen Capillovirus (ASGV) und Apple Stem Pitting Foveavirus (ASPV) auf Kernobst und Auswertung der Reaktionen einiger commertial Sorten zu den Krankheiten. Während der Untersuchung wurden 619 Proben von 134 gesammelt und Obstgärten, 18,41% der Proben als ASPV, ASGV oder ASPV wurden gegründet + ASGV durch ELISA-Test infiziert. Die Häufigkeit der Erkrankungen wurden als 10,34% ASPV, 5,49% und 2,58% ASGV ASPV + ASGV bestimmt. Die Infektionsrate der Arten wurden als 19,35, 18,33 und 17,39% in Birne, Apfel und Quitte in jeweils bestimmt. Das Vorhandensein dieser Krankheiten wurde auch von molekularbiologischen Mitteln bestätigt. Durch die Beobachtung der macroskobik Krankheiten in Untersuchungsgebiet die Studie zeigte, dass Krankheiten induzieren einige Symptome an der Wirtspflanze especialy ASPV wurde resultierenden wirtschaftlichen Schaden auf Quitte und Birne. ASPV und ASGV wurden ermittelt, kann unterschiedliche biologische Aktivität auf ihren Wirtspflanzen nach ihrer Herkunft die haben. Auswirkungen der Krankheiten Agenten waren sehr wertvoll für den Fall des Seins Mischinfektion auf den Indikator Pflanzen. Es wurden jedoch keine signifikante Unterschiede zwischen Isolaten durch die molekulare Charakterisierung beobachtet worden. Darüber hinaus wurden die Krankheiten gegründet, dass sie den Stammdurchmesser und Trockengewicht der holzigen Gewebe reduzieren, und erhöht den Winkel der Filialen auf einigen commertial Sorten. Die floem Nekrose und Stiel Einstechen Symptome bei einigen succeptible commertial Sorten wurden in der Reaktion Assay aufgrund der ASPV und ASGV Infektionen beobachtet.


Keywords : Turkish ASPV
English ASPV
German ASPV
Turkish ASGV
English ASGV
German ASGV
Turkish elma
English apple
German Apfel
Turkish armut
English peare
German Birne
Turkish ayva
English quince
German Quitte


Coverage : World


Structure : Atomic


Aggregation Level : Level 2


Version : Turkish Adana, 2009


Status : Final


Contribute : Role : Author
Date : 2009-06-30
name : Nevzat Birişik
e-mail :
organization : Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı. 01330 Adana, Turkey




Identifier : Catalog : URI
Entry : http://traglor.cu.edu.tr/common/object_xml.aspx?id=1553


Contribute : Role : Undefined
Date : 2013-09-08
name :
e-mail :
organization :


Metadata Schema : TrAgLor LOM AP


Language : Turkish Turkish
Format : Text


Requirements : Operating System: Multios
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Size : 5092419 bytes


Location : http://traglor.cu.edu.tr/objects/objectFile/HqDqa59V-892013-23.pdf


Interactivity Type : Expositive


Learning Resource Type : Thesis


Interactivity Level : Very Low


Semantic Density : High


Intended End User Role : Learner


Context : University Second Cycle


Typical Age Range : English 18U


Difficulty Level : Difficult


Duration : Year : 0 Month : 0 Day : 0 Hour : 0 Minutes : 0


Description :


Cost : No


Copyright and Other Restrictions : Yes


Description : This resource is licensed under the license(CC-BY-NC-ND) Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported


Kind : IsVersionOf


Resource : Catalog : URI
Entry : http://library.cu.edu.tr/tezler/7350.pdf


Description : Turkish Çukurova Üniversitesi Kütüphanesi Tez Koleksiyonu. 01330 Adana
Çukurova Üniversitesi Library, Thesis Collection. 01330 Adana, Turkey


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e-mail :
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Purpose : Discipline


Source : English AGRICOLA


Entry : Viral


Description :


Keywords : Turkish yumuşak çekirdekli meyveler
English pome fruits
Turkish bitki hastalıkları
English plant diseases