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Characterization Of Some Watermelon Genotypes By SSR And SRAP Markers And Evaluation For Fusarium Wilt (Fusarium oxysporum f.sp. Niveum) With Molecular And Classical Techniques

Identifier : Catalog : URI
Entry : http://traglor.cu.edu.tr/objects/objectFile/CW1MiTio-1592013-0.pdf


Title : Turkish Bazı Karpuz Genotiplerinin SSR ve SRAP Markörleri İle Karakterizasyonu ve Fusarium Solgunluğu (Fusarium oxysporum f.sp. Niveum)'na Dayanımlarının Klasik ve Moleküler Yöntemlerle Araştırılması
English Characterization Of Some Watermelon Genotypes By SSR And SRAP Markers And Evaluation For Fusarium Wilt (Fusarium oxysporum f.sp. Niveum) With Molecular And Classical Techniques
German Charakterisierung von einigen Wassermelone-Genotypen von SSR und SRAP Marker und Auswertung für Fusarium Wilt (Fusarium oxysporum f.sp. Niveum) mit molekularen und klassische Techniken


Language : Turkish Turkish



Descriptions : Turkish Bu çalışmada, Çukurova Üniversitesi Bahçe Bitkileri Bölümü karpuz genetik kaynak koleksiyonunda yer alan toplam 93 genotip arasındaki genetik çeşitlilik SSR ve SRAP markörleri ile değerlendirilmiştir. Araştırmada, 14 SSRprimeri ve 31 SRAP primer kombinasyonu kullanılmıştır. En yüksek polimorfizm oranı, % 100 ile SSR markörlerinden elde edilmiş ve bunu % 97.3 ile SRAP markörleri izlemiştir. Moleküler karakterizasyon sonucu elde edilen verilerle yapılan kümeleme (cluster) analizlerine göre Türkiye'nin değişik bölgelerinden toplanan Citrullus lanatus var. lanatus alt türüne ait karpuz genotiplerinin genetik olarak birbirine yakın olduğu ve birlikte kümelendiği tespit edilmiştir. Bu sonuç temel koordinat analizleri ile de desteklenmiştir. Fusarium solgunluğu (Fusarium oxysporum f.sp. niveum)'na karşı dayanımın klasik yöntemle değerlendirilmesiyle elde edilen ortalama hastalık oluşum düzeyi, ırk 0 için % 25.9, ırk 1 için % 39.4 ve ırk 2 için ise % 67.0 olarak bulunmuştur. 1 no'lu ırk için RAPD primeri OPP01 ile yapılan moleküler taramada 700 bp bandı, yalnızca Kar 26 ve PI 482293 genotiplerinde elde edilmiştir.
English In this study, the genetic diversity of 93 genotypes selected from the watermelon genetic resource collection of Horticulture Department in Çukurova University was evaluated by SSR and SRAP markers. Fourteen SSR primers and 31 SRAP primer combinations were used in the experiment. The highest polymorphism with 100 % was obtained from SSR markers, followed by SRAP markers with 97.3 %. According to cluster analysis performed using molecular characterization data set, it was determined that watermelon genotypes collected from the different regions of Turkey of which belong to Citrullus lanatus var. lanatus subspecies were genetically close and grouped together in the same cluster. This result was supported by principle coordinate analyses as well. The mean disease incidence obtained by classical method to evaluate the resistance to fusarium wilt (Fusarium oxysporum f.sp. niveum) was 25.9 % for race 0, 39.4 % for race 1 and 67.0 % for race 2. In the molecular analyses for determining resistance to race 1 with the RAPD primer OPP01, 700 bp band was recorded only in Kar 26 and PI 482293.
German In dieser Studie wurde die genetische Vielfalt der 93 Genotypen aus der Wassermelone genetische Ressource Sammlung Gartenbau-Abteilung an der Çukurova Universität ausgewählt von SSR und SRAP Marker evaluiert. Im Experiment wurden vierzehn SSR Zündkapseln und 31 SRAP Grundierung Kombinationen verwendet. Die höchste Polymorphie mit 100 % wurde von SSR Markierungen, gefolgt von SRAP Marker mit 97,3 % erhalten. Laut Clusteranalyse mit molekularen Charakterisierung Datensatz durchgeführt wurde festgestellt, dass Wassermelone Genotypen zusammengestellt aus die verschiedenen, die Regionen der Türkei davon Citrullus Lanatus var. Lanatus Unterart angehören genetisch enge und zusammen in demselben Cluster gruppiert wurden. Dieses Ergebnis wurde durch Prinzip koordinieren Analysen sowie unterstützt. Die mittlere Krankheitsinzidenz, die durch klassische Methode zur Bewertung der Resistenz gegen Fusarium Wilt (Fusarium Oxysporum f.sp. Niveum) war 25,9 % für Rennen 0, 39,4 % für Rennen 1 und 67,0 % für Rennen 2. In die molekularen Analysen zur Bestimmung der Beständigkeit gegen 1 grundiert RAPD OPP01 Rennen wurde 700 bp-Band nur in Kar-26 und PI-482293 aufgenommen.


Keywords : Turkish Citrullus lanatus
English Citrullus lanatus
German Citrullus lanatus
Turkish polimorfizmp
English polymorphismp
German Polymorphismus
Turkish moleküler markörler
English molecular markers
German molekulare Marker
Turkish genetik kaynaklar
English genetic resources
German genetische Ressourcen
Turkish Fusarium oxysporum f.sp. niveum
English Fusarium oxysporum f.sp. niveum
German Fusarium oxysporum f.sp. niveum


Coverage : Turkey


Structure : Atomic


Aggregation Level : Level 2


Version : Turkish Adana, 2010


Status : Final


Contribute : Role : Author
Date : 2013-01-01
name : İlknur Solmaz
e-mail :
organization : Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Bahçe Bitkileri Anabilim Dalı. 01330 Adana - Turkey




Identifier : Catalog : URI
Entry : http://traglor.cu.edu.tr/common/object_xml.aspx?id=1697


Contribute : Role : Undefined
Date : 2013-09-15
name :
e-mail :
organization :


Metadata Schema : TrAgLor LOM AP


Language : Turkish Turkish
Format : Text


Requirements : Operating System: Multios
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Duration : Year : 0 Month : 0 Day : 0 Hour : 0 Minutes : 0


Size : 2395791 bytes


Location : http://traglor.cu.edu.tr/objects/objectFile/CW1MiTio-1592013-0.pdf


Interactivity Type : Expositive


Learning Resource Type : Thesis


Interactivity Level : Very Low


Semantic Density : High


Intended End User Role : Learner


Context : University Second Cycle


Typical Age Range : English 18U


Difficulty Level : Difficult


Duration : Year : 0 Month : 0 Day : 0 Hour : 0 Minutes : 0


Description :


Cost : No


Copyright and Other Restrictions : Yes


Description : This resource is licensed under the license(CC-BY-NC-ND) Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported


Kind :


Resource : Catalog : URI
Entry : http://library.cu.edu.tr/tezler/7818.pdf


Description : Turkish Çukurova Üniversitesi Kütüphanesi, Tez Koleksiyonu. 01330 Adana.
English Çukurova University Library Thesis Collection. 01330 Adana, Turkey


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e-mail :
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Purpose : Discipline


Source : English AGRICOLA


Entry : Plant Breeding and Genetics


Description :


Keywords : Turkish moleküler genetik
English molecular genetics
Turkish karpuz hastalıkları
English watermelon diseases