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Determination Of Trasformation Efficients Of CM-ADH Genes Responsible For Aroma Synthesis In Melon (Cucumis melo L., var. cantalupensis Naud)

Identifier : Catalog : URI
Entry : http://traglor.cu.edu.tr/objects/objectFile/nxgr9I4r-1592013-14.pdf


Title : Turkish Kavun'da (Cucumis melo L., var. cantalupensis Naud) Aroma Sentezinden Sorumlu CM-ADH Genlerinin Transformasyon Etkinliklerinin Araştırılması
English Determination Of Trasformation Efficients Of CM-ADH Genes Responsible For Aroma Synthesis In Melon (Cucumis melo L., var. cantalupensis Naud)
German Bestimmung der Trasformation Koeffizienten von CM-ADH Gene verantwortlich für Aroma Synthese In Melone (Cucumis melo L., var. cantalupensis Naud)


Language : Turkish Turkish



Descriptions : Turkish Yapılan bu çalışma ile kavunda aroma sentezinden sorumlu (Cucumis melo L., var cantalupensis Naud) olan Cm-ADH genleri Agrobacterium tumefaciens aracılığıyla Vedrantais kavun çeşidine aktarılmıştır. Escherichia coli (DH5?)' den Agrobacterium tumefaciens' e aktarılmış ticari rekombinant RNAi - pFGC5941 plasmidi kullanılmıştır. Gen aktarımı olan bitkilerden DNA izolasyonu ve PCR yapılmıştır. Jel elektroforezi sonucunda elde edilen bantlara UVP jel dökümantasyon sistemiyle bakılmış, istenilen bölgelerde bantlar görülmüştür.
English In this research, Cm-ADH genes responsible from synthesis of aroma volatiles in melon (Cucumis melo L., var cantalupensis Naud) were transformed by Agrobacterium tumefaciens, for that, commercial recombinant RNAi - pFGC5941 plasmid which was transformed from Escherichia coli (DH5?) to Agrobacterium tumefaciens, was used. After transformation and regeneration, the integration of the transgenes in the plant genom was confirmed by PCR, Gel Electrophoresis and UV documentation system after DNA isolation. Bands were seen in the intended regions.
German In dieser Untersuchung, Cm-ADH Gene aus Synthese von Aroma, Volatiles in Melone (Cucumis Melo L., Var Cantalupensis Naud) durch Agrobacterium Tumefaciens, dafür transformiert wurden, diente kommerzielle rekombinante RNAi - pFGC5941-Plasmid, das von Escherichia coli (DH5?) in Agrobacterium Tumefaciens, verwandelt wurde. Nach Transformation und Regeneration die Integration von transgenen in das Genom der Pflanze durch PCR, Gelelektrophorese und UV-Dokumentationssystem nach DNA Lokalisierung bestätigt. Bänder wurden in die vorgesehenen Regionen gesehen.


Keywords : Turkish Agrobacterium tumefaciens
English Agrobacterium tumefaciens
German Agrobacterium tumefaciens
Turkish RNAi
English RNAi
German RNAi
Turkish Cucumis melo
English Cucumis melo
German Cucumis melo
Turkish aroma
English aroma
German Aroma
Turkish transformasyon
English transformation
German Transformation


Coverage : World


Structure : Atomic


Aggregation Level : Level 2


Version : Turkish Adana, 2009


Status : Final


Contribute : Role : Author
Date : 2009-01-01
name : Esra Kocaman
e-mail :
organization : Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Bahçe Bitkileri Anabilim Dalı. 01330 Adana - Turkey




Identifier : Catalog : URI
Entry : http://traglor.cu.edu.tr/common/object_xml.aspx?id=1703


Contribute : Role : Undefined
Date : 2013-09-15
name :
e-mail :
organization :


Metadata Schema : TrAgLor LOM AP


Language : Turkish Turkish
Format : Text


Requirements : Operating System: Multios
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Duration : Year : 0 Month : 0 Day : 0 Hour : 0 Minutes : 0


Size : 852167 bytes


Location : http://traglor.cu.edu.tr/objects/objectFile/nxgr9I4r-1592013-14.pdf


Interactivity Type : Expositive


Learning Resource Type : Thesis


Interactivity Level : Very Low


Semantic Density : High


Intended End User Role : Learner


Context : University Second Cycle


Typical Age Range : English 18U


Difficulty Level : Difficult


Duration : Year : 0 Month : 0 Day : 0 Hour : 0 Minutes : 0


Description :


Cost : No


Copyright and Other Restrictions : Yes


Description : This resource is licensed under the license(CC-BY-NC-ND) Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported


Kind : IsVersionOf


Resource : Catalog : URI
Entry : http://library.cu.edu.tr/tezler/7487.pdf


Description : Turkish Çukurova Üniversitesi Kütüphanesi, Tez Koleksiyonu. 01330 Adana.
English Çukurova University Library Thesis Collection. 01330 Adana, Turkey.


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e-mail :
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Date :


Description :


Purpose : Discipline


Source : English AGRICOLA


Entry : Plant Breeding and Genetics


Description :


Keywords : Turkish kavun ıslahı
English melon breeding
Turkish kavun genetiği
English melon genetics
Turkish moleküler genetik
English molecular genetics