TrAgLor - Turkish Agricultural Learning Objects Repository

Object Summary

Determination Of Trasformation Efficients Of CM-ADH Genes Responsible For Aroma Synthesis In Melon (Cucumis melo L., var. cantalupensis Naud)

Title of object

Determination Of Trasformation Efficients Of CM-ADH Genes Responsible For Aroma Synthesis In Melon (Cucumis melo L., var. cantalupensis Naud)

Object description

In this research, Cm-ADH genes responsible from synthesis of aroma volatiles in melon (Cucumis melo L., var cantalupensis Naud) were transformed by Agrobacterium tumefaciens, for that, commercial recombinant RNAi - pFGC5941 plasmid which was transformed from Escherichia coli (DH5?) to Agrobacterium tumefaciens, was used. After transformation and regeneration, the integration of the transgenes in the plant genom was confirmed by PCR, Gel Electrophoresis and UV documentation system after DNA isol...

Language of object

Turkish

Author(s) / Publisher

Esra Kocaman

Discipline(s)

Plant Breeding and Genetics

Keywords

Agrobacterium tumefaciens, RNAi, Cucumis melo, aroma, transformation

Technical format

Text

Intended end-user role

Learner

Learning resource type

Thesis

Typical age range

18U

Copyright - Restrictions

This resource is licensed under the license(CC-BY-NC-ND) Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported

Cost with use

No

Source code available

No


Annotations on the Object


Comments / Feedback / Suggestions







Please enter the result of the operation above in this box.